FastQCFastQC Report
Thu 28 Apr 2016
ERR1042362.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042362.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5556383
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT154250.27760865296722703No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT123070.22149301083096684No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT106070.19089756771626434No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAAGA11200.018.1696412
CTAGTAC2550.018.1372553
ACGGACC11250.016.9377778
GACGGAC11800.016.4618647
ATTAGAC5900.015.9915263
AAGACGG12700.015.8779545
TCTAGCG6200.015.21774228
CGGACCA12800.015.1757829
GGTATCA108750.015.0381611
TTAACGG5000.014.79999935
GCGCAAG14150.014.7738511
AATCGTC4900.014.7244928
GTATTAG22600.014.6526551
TTAGACT6700.014.6343294
CGTTATT9450.014.4867732
AGACGGA13550.014.4723246
TCTATAC7350.014.3469393
GTACAAG9500.014.215791
TATACTG5950.013.9915965
TAACCGG3050.013.95081922
Produced by FastQC (version 0.11.5)