FastQCFastQC Report
Thu 28 Apr 2016
ERR1042361.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042361.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14164442
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT342460.24177443770817092No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT282020.1991042075642655No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT237920.16796990661545297No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA186100.016.9691031
ACGGACC35850.016.4616478
GACGGAC35300.016.4036857
AAGACGG38300.015.6984345
TCGTTTA25300.014.91699630
CGGACCA39600.014.8093439
CGCAAGA41900.014.4379482
AGACGGA40950.014.2307696
CGAACGA21600.013.87516
TCTAGCG22200.013.83333328
CGTTTAT27850.013.61759431
CAAGACG48300.013.5973084
TAACGCC29000.013.5879324
GCGCAAG47300.013.4545451
TTAACGG6750.013.42962935
TACGACG24650.013.2089255
CGAGCCG39200.013.02551115
ATACCGC37850.013.00132127
CTAGCGG24150.012.9461729
ATAACGC32750.012.7664133
Produced by FastQC (version 0.11.5)