FastQCFastQC Report
Thu 28 Apr 2016
ERR1042360.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042360.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7671497
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT173980.22678754876655755No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT134070.1747638042483755No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT118370.15429843744969202No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA101650.016.9621261
CGAACGA11600.016.58620616
AAGACGG15200.014.7269745
ACGAACG13300.014.60526415
TAACGAA13600.014.55514713
ACGGACC14650.014.3959058
GACGGAC14650.014.1433457
CGTTATT13750.013.8581812
CTAGTAC5350.013.8317763
GCGTTAT16600.013.8192771
TATACTG9800.013.7806135
ATAACGA14400.013.74652812
TTAACGG2700.013.70370435
AACGAAC14350.013.66550514
TCTATAC8050.013.3291933
TCCGATA15200.013.2664488
CCGATAA15200.013.2664489
CGATAAC15450.013.05178110
GTATTAG18900.012.9206351
TACCGTC11600.012.9181037
Produced by FastQC (version 0.11.5)