FastQCFastQC Report
Thu 28 Apr 2016
ERR1042359.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042359.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16442112
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT430750.2619797262054899No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT331290.20148871385865758No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT303620.18465997555545177No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC176360.10726115963691284No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA176200.10716384853721955No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT164960.10032774378376695No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA180750.020.9513131
TCTAGCG29150.017.4528328
AAGACGG43050.016.9314755
ACGGACC40500.016.6271618
CTAGCGG30600.016.62581629
GACGGAC41050.016.5395857
CGCAAGA47600.015.0798312
CAAGACG51350.014.9513154
CGGACCA45600.014.8486849
AGACGGA47200.014.7372896
TCGTTTA32500.014.629230530
GTATCAA264200.014.2566242
TATACTG23300.014.2124465
CGCAATA36850.014.20759936
GCGCAAG52250.014.162681
CGGTCCA37600.014.07180810
CGAACGA28450.013.5905116
CGTTTAT35350.013.55445531
TAACGCC37950.013.5520424
TAGCGGC38400.013.53776130
Produced by FastQC (version 0.11.5)