FastQCFastQC Report
Thu 28 Apr 2016
ERR1042358.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042358.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20284083
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT877230.4324721014008866No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT689690.34001537067265997No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT625950.30859171696349297No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT292150.14402918781194102No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA346600.017.6193031
AAGACGG46300.015.3833695
GACGGAC43800.015.2054817
ACGGACC44100.015.0600918
TATACTG29900.014.7876265
TCTAGCG31450.014.52941128
GTAAACG33950.014.11340127
TCGTTTA38350.013.8930930
CTAGCGG32750.013.83969429
AGACGGA51000.013.675496
CGAACGA30000.013.62833316
GTATTAG59400.013.5168351
TAAACGC35650.013.44039328
CGCAAGA53250.013.1671352
TAACGCC44650.012.7614774
CGCATCG42650.012.75263813
CAAGACG60500.012.6595034
CGTTTAT42350.012.624557531
CGGACCA54000.012.5731499
TTAACGG10600.012.56603835
Produced by FastQC (version 0.11.5)