FastQCFastQC Report
Thu 28 Apr 2016
ERR1042357.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042357.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3317718
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG95780.28869240845665606No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC93980.2832669925533153No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA55600.1675850690143044No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT55060.16595744424330217No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG54780.16511349065833805No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT49860.1502840205225399No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA42620.12846179211132472No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC41590.12535724856663524No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT41020.123639200197244No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT37540.11315006278411847No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT36970.11143201441472722No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA35720.1076643644818517No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC34020.10254036057314093No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC33370.10058118260804566No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGACG8650.019.4624274
GACGGAC8850.018.186447
AAGACGG9450.018.0105825
ACGGACC9150.017.9945358
TCGTTTA6400.017.05468830
TAAACGC7800.016.83974528
CGCATCG6950.016.50359713
CGCAAGA10350.015.9082132
TAGAGTG5950.015.8571425
CGGACCA10450.015.75598059
CCGGTCG6850.015.66423320
GTCCTAA2600.015.6538451
GCGCAAG10800.015.5879631
TACGACG9050.015.5359125
GTAAACG8100.015.53086427
CGAGCCG11350.015.48458115
AGACGGA10950.015.3744296
TCGAACG3750.015.2933323
CGAACGT3650.015.2054814
ATTGACG10250.014.98048932
Produced by FastQC (version 0.11.5)