FastQCFastQC Report
Thu 28 Apr 2016
ERR1042356.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042356.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5072545
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT162430.32021401485841916No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT139130.27428046473712897No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT122670.24183127010208877No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60020.11832324799484283No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA52170.10284778153766994No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC51740.10200008082727705No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG12300.017.8983755
ACGGACC11750.017.6340438
TCTAGCG9450.017.61904728
GACGGAC11750.017.3191497
CTAGCGG10050.016.93532429
CGCAAGA13700.016.609492
ATTAGAC3400.016.3235283
CGGACCA13000.016.0807699
CAAGACG15200.015.9440794
TATACTG7700.015.6168825
AGACGGA13350.015.5205996
CGAACGA8300.015.379518516
ATTAGAG9350.015.2352953
TAACGGC3400.015.23529336
GTATTAG16000.015.1468741
TTAACGG3300.015.13636435
TCTATAC6850.015.1240883
GCGCAAG14850.014.9494941
TTAGACT4550.014.63736254
TCGCTAA2151.2732926E-1114.62790714
Produced by FastQC (version 0.11.5)