FastQCFastQC Report
Thu 28 Apr 2016
ERR1042355.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042355.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3971820
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT236360.5950924261421716No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT213790.53826709166075No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT176070.44329803465413836No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88950.22395274710334304No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC43560.10967264377539769No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA42870.10793540492771575No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGG2100.023.78571335
CTAACGC759.2751725E-619.7333343
TAACGGC2450.019.63265236
TCTAGCG5650.016.69911628
TATACTG6100.016.073775
GTACTAG2650.016.0566041
AAGACGG9350.016.026745
CTAGCGG5900.015.99152629
GACGGAC9450.015.6613747
CGCAAGA9950.015.432162
TAATCCG855.368272E-415.2352945
ACGGACC9850.015.2131978
TCGAACG3300.015.1363643
CGGTCCA6900.014.74637610
CGAACGA5800.014.67241416
TTTAGCG2151.2732926E-1114.62790726
GCGCAAG10600.014.4858491
TCGTTTA5900.014.42372930
AGACGGA10650.014.417846
GTATTAG13800.014.3442021
Produced by FastQC (version 0.11.5)