FastQCFastQC Report
Thu 28 Apr 2016
ERR1042354.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042354.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9196739
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT243740.2650287237682835No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT186800.20311547386524723No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT186580.2028762586390676No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC110380.12002080302594215No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA109520.11908568895996723No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGG5450.017.65137735
GGTATCA124300.017.3093341
TAACGGC5850.016.44444536
TCTAGCG15850.015.75709728
CTAGCGG15650.015.72204529
TCGTTTA14300.015.52447530
TAAACGC13350.015.10486828
AAGACGG19450.015.0282775
GTAAACG13650.014.77289427
CGAACGA13500.014.52592716
ACGGACC19300.014.1865278
GTATTAG32700.014.03058051
CGTCTAA2800.013.8751
ACGCTTC14950.013.85953331
GACGGAC20100.013.621897
GTATACG3600.013.3611111
CGCAATA19300.013.22797936
CGGTCCA19450.013.2210810
CGTTTAT17150.013.1603531
TATACTG17400.013.0775855
Produced by FastQC (version 0.11.5)