FastQCFastQC Report
Thu 28 Apr 2016
ERR1042353.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042353.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5406864
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT306160.5662432049335807No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT296460.5483030459060927No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT232000.4290842159151775No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123180.22782152463979122No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC95230.17612797362759633No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG88920.16445762275507578No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA58310.1078443992673017No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGG3100.019.69354835
TAACGGC3600.016.95833436
TCGTTTA9450.016.44444530
TCTAGCG8400.015.63690428
TAACGCC11000.015.3045444
AAGACGG13400.015.1865665
TCGCTAA2201.8189894E-1215.13636414
ACGGACC12500.015.0959998
CTAGCGG8700.014.88505829
GACGGAC12750.014.8000017
GTATTAG18800.014.7606391
ATAACGA8050.014.24844712
GTCGTAC1304.451009E-614.2307691
CGAACGA7950.014.19496816
CGTTTAT10950.014.19178231
TCGCCGG11250.014.14222217
CGCATCG10900.014.08715513
ATTAGAG10050.013.990053
ATGGTCG10850.013.98156736
CAAGACG16450.013.8328264
Produced by FastQC (version 0.11.5)