FastQCFastQC Report
Thu 28 Apr 2016
ERR1042352.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042352.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3825536
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT221290.5784548884130224No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT209170.5467730535015224No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT168740.4410885167464114No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80940.21157819453274No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA47600.12442700839830026No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC45880.11993090641416No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC39260.10262614180078294No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGTC3000.017.26666628
TCTAGCG5750.017.05217428
TTAACGG2950.016.93220335
CGTTCGG1107.81929E-716.81818224
AAGACGG8200.016.4695115
ACGGACC7650.016.4444458
TCGTTTA6200.016.4112930
TAACGGC3050.016.37704836
GTACGTA3500.015.85714313
CGGACCA8050.015.8571439
CTAGCGG6200.015.81451529
GACGGAC7950.015.5911947
TAACGCC7300.014.9520554
CGGTCCA6700.014.91044810
TCGCCGG7200.014.90277817
TCGTCCC3600.014.90277830
TCTATAC4600.014.8804353
TCTACGC750.00410672914.83
GTGTACG1252.9629227E-614.7999991
ACGAATA1252.9629227E-614.7999993
Produced by FastQC (version 0.11.5)