FastQCFastQC Report
Thu 28 Apr 2016
ERR1042351.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042351.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1807627
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC30800.1703891344840501No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA28370.15694609562702924No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC25820.14283920299929134No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC19450.10759963200372644No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT18460.10212283839531053No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATACG250.00549716629.635
GGTATCA15200.020.690791
TCGTTTA3300.017.37878830
ACTAGGT650.00158040717.07692332
TACGGGT803.3839062E-416.18754
GTATAGA1951.8189894E-1216.1282061
AATACTG2250.015.6222225
TACGCTA2055.456968E-1215.3414639
ATTAGAC1455.3505573E-815.3103453
CGTTTAT3750.015.29333231
GACGGAC4000.015.2625017
AACCGAA855.366263E-415.23529315
AATACGC855.366263E-415.2352935
ATACCGA855.366263E-415.2352936
ACGGACC3850.014.8961048
ATACCGT3850.014.8961046
TAACGCC4150.014.7108434
CTAGCGG2900.014.67241429
CGCATCG4300.014.62790713
AGACGGA4300.014.6279076
Produced by FastQC (version 0.11.5)