FastQCFastQC Report
Thu 28 Apr 2016
ERR1042350.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042350.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9909372
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT305580.30837473858081016No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT220290.22230470306291863No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT215060.2170268711276557No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA94700.023.8722291
CTAGCGG14550.016.65635929
TCTAGCG14400.016.57291628
TCGTTTA14250.016.35789530
TATACCG3000.016.0333335
ACGGACC17400.015.6293118
GTATCAA148900.015.0832772
GACGGAC18050.015.0664827
AAGACGG21750.014.8850575
CGAACGA10950.014.8675816
CGCAATA15950.014.84639536
ATAACGC19450.014.0771213
TACCGTC12050.013.9709547
TAACGCC17400.013.8218394
TATACTG20350.013.7272735
CCGGTCG17800.013.51123520
GTATTAG25750.013.3631071
CGTTTAT17500.013.32000131
CAAGACG25000.013.2464
AGACGGA22200.013.1666676
Produced by FastQC (version 0.11.5)