FastQCFastQC Report
Thu 28 Apr 2016
ERR1042349.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042349.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6305674
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT337300.5349150622122234No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT271880.4311672312904219No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT234840.3724264844646266No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133190.21122246408552042No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA80960.1283923019172891No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT76820.12182678647833681No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC74970.1188929208836359No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG18300.018.196725
GACGGAC19550.016.2762177
ACGGACC20000.016.0958
CAAGACG23300.015.641634
CGCAAGA21700.015.51612952
GCGCAAG22500.015.0466671
AGACGGA22150.014.6997756
CGGACCA22450.014.6681529
CGAGCCG19850.014.63224215
TATACCG1803.3396645E-914.3888885
TCTAGCG12150.014.16049428
GTAAACG10350.014.12077227
TATACTG7400.014.05
CTAGTAC3350.013.805973
GTATTAG21050.013.79809951
TAAACGC10750.013.76744228
TCGTTTA13100.013.69847430
CTAGCGG12850.013.53307329
TACCGTC13750.013.4545467
TAATACG3900.013.2820524
Produced by FastQC (version 0.11.5)