FastQCFastQC Report
Thu 28 Apr 2016
ERR1042348.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042348.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8257018
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT430560.5214473312278113No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT366860.444300835967658No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT313050.3791320304739556No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167610.20299095872141734No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA123090.14907318840772782No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC113690.1376889332202013No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT104810.1269344453409209No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA90800.10996706074759678No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC89390.10825942246946779No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGG87720.10623690053721574No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG87510.10598257143196249No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG86080.10425071133428557No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG17600.017.55397828
CTAGCGG18450.016.8455329
AAGACGG23750.016.6694745
ACGGACC24400.015.3913938
GACGGAC24400.015.2397547
CGCAATA20950.015.18854536
CAAGACG28050.015.169344
TCGTTTA17550.015.07407430
TTAACGG4050.015.07407435
CGAACGA17450.014.73638816
GTATTAG24950.014.6813631
ATAACGA18150.014.57575712
TAACGGC4200.014.53571436
TAGCGGC21700.014.49308830
CGGTCCA22000.014.46363510
CGGACCA26850.014.40037259
ACGAACG18150.014.37190115
GGTATCA228700.014.3097941
AGACGGA27150.014.1731126
CGCAAGA26950.014.0723562
Produced by FastQC (version 0.11.5)