FastQCFastQC Report
Thu 28 Apr 2016
ERR1042347.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042347.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6644416
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT300030.4515521002899277No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT249790.37593973646442364No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT225860.3399245321183984No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118550.17842049624827824No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA103060.15510768741752473No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC97880.14731166742118496No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA78260.11778311291767403No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT77630.1168349483235246No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG76660.11537507585316753No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG71890.10819611535460753No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC69270.10425295466147816No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGG66760.10047534651653359No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG18300.017.6912565
ACGGACC19200.016.5729168
GACGGAC19750.016.0177217
CAAGACG21700.015.6866364
CGGACCA20900.015.4904319
CGCAAGA21150.015.3073272
TCGTTTA13800.015.28260930
AGACGGA21350.015.2505866
TTAACGG2800.015.19642835
GCGCAAG22100.014.8167421
TCTAGCG14250.014.67017628
TAAACGC13150.014.06844128
CTAGCGG15000.014.06000129
CGCATCG15100.013.72185513
GGTATCA179350.013.7086711
GTAAACG13250.013.68301927
AACGCCG15850.013.5394325
TAACGCC15750.013.50793654
TCTATAC7700.013.4545453
ACGCTTC14100.013.38297831
Produced by FastQC (version 0.11.5)