FastQCFastQC Report
Thu 28 Apr 2016
ERR1042346.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042346.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4678467
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT191260.40880912486932147No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT165260.35323536534510125No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT145980.3120252852056026No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA82080.17544208391338445No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC81270.17371074755897603No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73950.15806459680061866No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA61570.13160293745793228No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG60460.12923036541670593No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT58330.12467759204029868No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG56460.1206805562591336No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC56090.11988969891205815No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC55670.11899196895051307No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGG51140.10930931007956239No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT47680.1019137251582623No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG13200.019.481065
TCTAGCG10450.017.88038328
CTAGCGG10650.017.54460129
CAAGACG17200.016.779074
ACGGACC15250.016.7409848
GACGGAC15500.016.3516127
CGCAAGA16250.016.0523072
GCGCAAG16700.015.7305381
AGACGGA16450.015.6322186
TATACTG6650.015.5789485
CGGACCA16900.015.4349119
CGCAATA12350.015.42915136
CGGTCCA13000.015.36923110
CGAACGA10750.015.31627916
TAGCGGC12500.014.94800130
CCGGTCC13450.014.7174739
GCAATAC13500.014.52592737
ATAACGA11800.014.42372912
TAACGAA11550.014.41558513
CGCAACG908.280709E-414.38888912
Produced by FastQC (version 0.11.5)