FastQCFastQC Report
Thu 28 Apr 2016
ERR1042345.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042345.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5506354
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT238770.4336263160704888No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT219580.39877566898168915No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT188380.34211385610151474No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98140.17823045884808714No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC77220.1402379868784317No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA76740.13936626668027519No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA56470.10255424914562339No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG55980.10166436811000527No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGG3400.020.13235335
TCTAGCG9300.017.10752728
CTAGCGG9450.016.8359829
GACGGAC12300.016.5447167
ACGGACC12450.016.345388
AAGACGG13250.016.0566045
TATACTG7850.016.0254785
TAACGGC4300.015.91860436
CGGTCCA10800.015.07407310
CGCAAGA13500.014.9370372
CGGACCA13500.014.89
TATACCG1956.7484507E-1014.2307685
GTATTAG18600.014.2231181
AATCGTC3400.014.14705928
CGCTTAC1051.6574307E-414.0952381
TAACCCG11500.013.99565228
CGGGTAA11950.013.93305524
CCGGTCC11200.013.8750019
TCGTTTA9900.013.82828330
TCTATAC6850.013.7737233
Produced by FastQC (version 0.11.5)