FastQCFastQC Report
Thu 28 Apr 2016
ERR1042344.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042344.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2617610
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT308251.1776009413166972No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT302981.157468072019896No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT215680.8239577324353131No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125260.47852812298241526No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC52940.2022455598809601No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG49510.1891420035834215No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA41670.15919101776047617No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37920.1448649722456745No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT33280.12713887859535988No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32360.12362422209572854No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA32350.12358601930768907No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA31420.12003316002001825No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA30880.11797020946588681No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC29290.11189596616761092No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT28380.10841951245601904No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA28170.10761725390719015No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG6800.020.9485285
GACGGAC6600.019.6212127
ACGGACC6700.019.3283588
GTCTTAC2250.018.9111121
CGCAAGA7050.018.6312072
CAAGACG8250.018.1636374
CGGACCA7250.017.6068959
CGTCCTA953.6099482E-617.52631633
TATACCG953.6099482E-617.5263165
AGTACCG1705.456968E-1217.4117645
TCGAACG1950.017.0769233
GTATTAG8600.016.779071
AGACGGA8050.016.0869566
GCGCAAG8350.015.9520961
TAGACCG700.0025936815.85714325
AAGTGCG700.0025936815.8571437
CGTAGCG957.065183E-515.57894815
GCAAGAC9750.015.5589743
CGAACGT2150.015.4883724
CGCATCG5400.015.41666713
Produced by FastQC (version 0.11.5)