FastQCFastQC Report
Thu 28 Apr 2016
ERR1042343.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042343.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5738127
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT347170.605023207049966No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT308170.5370567782832273No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT243100.4236574059793379No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125210.21820709092008594No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA79610.13873865113128378No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC77810.13560173903435738No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG74300.1294847604453509No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT74040.1290316509202393No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC72230.12587731153388554No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA61650.10743923931972924No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGG4300.018.06976735
AAGACGG16950.017.6814175
TCGTTTA12800.016.91015630
ACGGACC17250.016.7304368
GACGGAC17550.016.3390317
TCTAGCG11600.016.26724228
CTAGCGG12000.015.87916729
TAACGGC4900.015.85714236
CGCAAGA19000.015.5789472
CGTTTAT14100.015.21985831
CGGACCA19550.015.0460379
ATGGTCG13850.014.96028936
GTATTAG21700.014.8341011
CAAGACG21850.014.8169344
AGACGGA19750.014.7999996
CGGTCCA13550.014.47232410
GCGCAAG20800.014.3197121
TATACTG5700.014.2807025
AGGCCCG18250.014.1917810
CGCATCG15150.014.16501713
Produced by FastQC (version 0.11.5)