FastQCFastQC Report
Thu 28 Apr 2016
ERR1042342.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042342.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6218152
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT118960.19131085891756908No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA101880.16384289094251797No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT99410.15987064967212125No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC98670.15868058548584854No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT82530.13272432066633302No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT79170.1273207859827164No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA75710.12175643181446835No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG71030.11423007993371664No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC67400.10839233264159512No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGG67220.10810285756925851No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC65390.10515986100050304No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG64520.10376073148420945No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG18350.018.550415
ACGGACC18550.017.652298
TCGTTTA12000.016.95833430
GACGGAC19150.016.9060047
CAAGACG21000.016.7380964
CGCAAGA20050.016.516212
CGGACCA19900.016.1758799
TCTAGCG12250.016.15918428
GGTATCA86100.016.0290371
AGACGGA20650.015.8571436
CTAGCGG13000.015.65384629
ATTGACG21000.015.24047732
TAACGCC14350.014.9547034
GCGCAAG22250.014.8831461
CGAACGA13450.014.85501916
CGTTTAT13750.014.79999931
TACCGTC15400.014.7759757
CGCATCG13800.014.74637713
CCGGTCG13950.014.72042920
TTGACGG22700.014.26211533
Produced by FastQC (version 0.11.5)