FastQCFastQC Report
Thu 28 Apr 2016
ERR1042341.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042341.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1134284
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT183781.6202291489609304No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT183101.6142341776838958No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT125071.1026339082628336No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69560.6132502971037236No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA24390.21502551389246433No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA23730.2092068653000483No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20860.1839045600572696No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA20330.17923200891487495No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT18670.16459722609152558No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17430.15366521964516822No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA14340.1264233648715842No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA13620.12007574822531218No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG13510.1191059734599095No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA13230.11663745587524818No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC13150.1159321651367735No Hit
GTACATGGGAAGCAGTGGTATCAACAAAAAAAAAAAAAAAAAA12650.11152409802130682No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC12230.10782132164431484No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11860.1045593519788695No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11630.10253164110575481No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCATT250.00549631429.59999811
GCGTTAG502.7020724E-422.1999991
CGTTAGA603.7262296E-521.5833342
GTTAGAC806.9599446E-720.81253
ACGTTTA851.2452783E-619.58823626
CGCAAGA1700.019.5882362
ATACCAT2350.018.8936166
TACGGTG801.61664E-518.528
CGTTATA701.21917925E-418.52
TAGGGGT801.61664E-518.54
GAACCTA609.236712E-418.56
CTTAATA500.007034827518.4999982
TGCGCGA500.007034827518.49999812
CTAGCGG1054.79713E-717.61904729
ACGGACC1603.45608E-1117.343758
GTATACT1401.8681021E-917.1785724
CCGTACA650.001579916517.07692332
CGTAAGA650.001579916517.07692332
GCCGTCG650.001579916517.07692310
TTATGCG1201.041135E-716.9583344
Produced by FastQC (version 0.11.5)