FastQCFastQC Report
Thu 28 Apr 2016
ERR1042340.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042340.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2114178
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT154040.7286046870225685No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT138210.6537292508010205No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT107400.5079988534551017No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52860.2500262513373992No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA27060.12799300721131332No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC26060.12326303650875185No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGG1303.274181E-1119.92307735
GCGTTAG701.2198122E-418.51
CCGGTTA500.007036788418.530
TAACGGC1451.546141E-1017.86206836
CGTAGCG1554.0199666E-1016.70967915
CGTGATA1251.6595368E-716.2824
GTTGACG700.002593459615.8571423
CGTGCGC957.064157E-515.57894810
TATTACG957.064157E-515.5789487
TCCGTAT1557.21775E-915.516129513
CGCACTA1557.21775E-915.516129529
CGAACGA4100.015.34146416
TAACGAA4250.015.23529413
GCGGACC855.366795E-415.23529414
CGCCGTA855.366795E-415.23529425
TCGTAGC1752.240995E-914.79999914
GCGGAAT1752.240995E-914.79999919
ACCGACC1904.5656634E-1014.6052648
CGAACGC1406.00121E-714.53571430
GTACGGA1406.00121E-714.5357146
Produced by FastQC (version 0.11.5)