FastQCFastQC Report
Thu 28 Apr 2016
ERR1042339.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042339.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3746330
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT323760.8642057693796329No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT299350.7990486689640262No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT228210.6091561608293984No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122060.3258121948680442No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA55170.1472641224878748No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC49950.1333304861023989No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA39000.10410188104091203No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38250.10209992179012527No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG37840.10100551739969518No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGTC3150.019.38095328
AAGACGG8150.017.4785275
TACGTAG3150.017.03174614
ACGGACC8600.016.9941868
GTACGTA3200.016.76562513
CGTTCGG904.4502718E-516.44444524
TATACTG3400.016.323535
TAGATCG803.3851175E-416.18755
CTAATCG3800.016.0657926
GTCTATA1851.8189894E-1116.01
GACGGAC8850.015.8870057
GTACTAG2250.015.6222221
ATGTACG3600.015.41666711
CGCAAGA9550.015.3036642
TAACGGC3050.015.16393436
AGACGGA9550.015.1099476
CGGACCA9750.014.9897439
TCGGTAA1001.0944115E-414.827
TACGTCG750.004106708314.85
TAGACTA4750.014.4105265
Produced by FastQC (version 0.11.5)