FastQCFastQC Report
Thu 28 Apr 2016
ERR1042338.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042338.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6861058
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT517540.7543151508120176No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT459960.6703922339674143No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT385590.5619978726313055No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT196250.2860346028265611No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA125000.18218764511245933No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC114470.16684015788818576No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG88280.12866820248422328No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA85010.12390217368808135No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG81540.11884464465975947No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC79770.11626486760496706No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC78390.11425351600292549No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT72140.10514413374730254No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGACC16600.017.8313268
GACGGAC16950.017.1356937
AAGACGG17600.017.1335225
TCTAGCG12500.016.42828
CGGACCA18150.016.2066129
CTAGCGG12850.016.12451429
CGCAAGA18400.015.9864132
AGACGGA19150.015.7467366
GCGCAAG20050.015.5935151
GTCGGGA11050.015.2352942
CTAACGC1101.4538813E-515.1363643
CGGTCCA14300.014.87762310
TTAACGG3250.014.835
GGTATCA265250.014.7232811
TAATACT18900.014.5846574
CAAGACG23150.014.0647954
TACGCTC800.006302829413.8755
TAACCGG2800.013.87522
CCGCCTA3350.013.8059715
GTATTAG22650.013.8035321
Produced by FastQC (version 0.11.5)