FastQCFastQC Report
Thu 28 Apr 2016
ERR1042336.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042336.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6606916
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT192550.2914370335569576No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT169240.25615582217179694No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT143230.21678798398526633No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA124160.18792429024373855No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC112640.1704880158912267No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA90740.13734093183567037No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC85330.12915254257811057No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC83830.12688219435512726No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG81450.12327990850799374No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT77340.11705915437701948No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG75340.11403202341304174No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGG72030.10902212166765855No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGG4850.017.54639235
AAGACGG18950.017.4749345
TCTAGCG14650.017.30034328
CTAGCGG15150.016.60726229
GACGGAC19200.016.2838547
ACGGACC19550.016.1815858
GGTATCA131400.015.5715381
CGCAAGA20900.015.4904312
CGGTCCA16300.015.43558410
TCGTTTA14750.015.42711930
GCGCAAG21500.015.4023251
AGACGGA20600.015.3567966
TAACGGC5550.015.33333336
CGGACCA20750.015.2457839
CCGGTCC17800.015.0702269
CGAACGA18150.014.77961416
ATAACGA18650.014.58176912
CGCAATA18000.014.49166736
TAACGAA18600.014.42204313
CAAGACG24700.014.3056684
Produced by FastQC (version 0.11.5)