FastQCFastQC Report
Thu 28 Apr 2016
ERR1042335.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042335.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20648898
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT899910.43581502509238024No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT689720.33402266794092356No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT672900.3258769547895486No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT349180.16910345530303844No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA307510.14892320161589254No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC294020.14239016532504545No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT245160.11872788562372674No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG240950.11668903589915548No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA217070.10512425408852326No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG207790.10063006752224744No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG57250.018.1930145
TCTAGCG41900.017.5286428
CTAGCGG42950.017.18626229
ACGGACC58400.017.0111318
GACGGAC58100.016.97167
AGACGGA63150.016.053846
CGGACCA63350.015.827949
TTAACGG15450.015.80582435
CGCAAGA64550.015.7629752
TAACGGC15650.015.48562336
GGTATCA471800.015.3277871
TCGTTTA50450.015.21803730
CAAGACG75650.015.0152024
CGGTCCA51550.014.64209610
CGCAATA52350.014.41833836
GCGCAAG71750.014.2843211
CGCATCG53950.014.2650613
TAGCGGC54950.014.07279430
TAACGCC56650.013.8464254
CGTTTAT56150.013.67319731
Produced by FastQC (version 0.11.5)