FastQCFastQC Report
Thu 28 Apr 2016
ERR1042334.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042334.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7866976
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT531340.6754056450661602No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT462110.5874048681475577No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT360470.4582065586573545No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200700.25511708692132784No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA118220.15027375194738107No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC108950.1384903169909251No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT102270.12999912545811756No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA95290.12112659298820794No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG89710.11403365155810823No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC88470.11245744235141938No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG85270.10838980568899663No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGG82030.10427132356829359No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC78940.1003435119161416No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGACC23100.017.4588748
GACGGAC23250.017.1075277
AAGACGG24900.016.4939775
TCTAGCG14450.016.0034628
CGGACCA25900.015.7142869
CAAGACG28200.015.6790784
CGCAAGA26550.015.3992462
AGACGGA26150.015.3518176
CTAGCGG15450.014.96763729
CGGTCCA15900.014.42767410
TATACTG11050.014.39819055
GCGCAAG28050.014.3778971
TCGTTTA16150.014.08978430
CGAGCCG23050.013.80477215
CGAACGA20600.013.65048516
CGCAATA17750.013.54929536
CGAAAGC29450.013.50594219
GCGTTAT20750.013.462651
GCGAAAG29350.013.42589418
CAGAGCG31000.013.30806514
Produced by FastQC (version 0.11.5)