FastQCFastQC Report
Thu 28 Apr 2016
ERR1042333.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042333.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3909265
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT981952.5118532511866043No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT873632.2347679167311503No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT538001.3762177800686317No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT385300.9856072688855834No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA141690.3624466491782982No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT107820.27580632164869867No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101260.25902567362407No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA99890.25552117853356066No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89640.22930141599507833No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA75410.1929007115148244No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73950.18916599411909912No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA67640.17302485249784805No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA51580.13194296114486995No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA49510.12664784812490326No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC47810.12229920458193548No Hit
GTACATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAA47200.1207388089576941No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA46980.12017604332272179No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45650.11677386925675287No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45640.11674828900061776No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG45110.11539253542545722No Hit
GAGTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAA43510.11129969444384047No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGACC7650.017.4117648
AAGACGG8750.016.9142865
GTACTAG1757.2759576E-1216.9142861
CGGACCA8200.016.4695139
TATACCG1708.54925E-1116.323535
CGCAAGA8300.016.2710842
ACGTTTA1403.4799086E-815.85714326
TAACGGG700.002593986715.8571434
TATACTG5600.015.8571435
GCGCAAG8650.015.8265891
GACGGAC8300.015.8253017
CGCGGTC2700.015.75925810
CGCTATA1557.225026E-915.51612952
CGGTCTA1201.9382987E-615.41666620
CAAGACG10250.015.3414634
TACACCG2750.014.85
AGTCGTA1152.212419E-514.47826114
CGAACGT2201.8189894E-1114.2954544
TCGAACG2201.8189894E-1114.2954543
GTACCGT800.006302189513.8756
Produced by FastQC (version 0.11.5)