FastQCFastQC Report
Thu 28 Apr 2016
ERR1042332.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042332.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7339245
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT443410.6041629622665546No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT396450.5401781790906285No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT246790.33626074616666973No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174260.2374358670408196No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT117430.16000283407898225No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC106990.14577793764889985No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA97540.13290195381132527No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGG82860.11289989637898722No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG80130.109180167714799No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA79860.10881228246229686No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGACC27400.018.2974458
AAGACGG28100.018.2366545
CGCAAGA28650.017.9511342
GACGGAC27300.017.8901127
CGGACCA28750.017.5669579
GCGCAAG30100.017.2707631
AGACGGA29650.016.784156
CAAGACG33650.016.3833584
GGTATCA202000.016.3386141
TATACTG11900.015.3907575
GGTTATC13950.015.1182792
GCAAGAC38650.014.69469453
TCGTTTA12400.014.47177530
CAGAGCG34850.014.33285414
GAGCGAA35200.014.08522716
GCGAAAG35350.014.02546118
ATTGACG25900.013.71428632
CGAAAGC36100.013.68282519
CGCGGTC9100.013.62087810
TTGACGG26400.013.52462133
Produced by FastQC (version 0.11.5)