FastQCFastQC Report
Thu 28 Apr 2016
ERR1042330.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042330.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6783282
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT670190.9880025627712367No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT668270.9851720745208588No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT300580.4431188324471841No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT280340.4132807688077836No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT78530.11576991786571751No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT72300.10658557317829334No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA68680.10124892345622664No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGGAC19050.015.9265097
AAGACGG20350.015.9090915
ACGGACC19000.015.8710538
CGCAAGA20050.015.6857862
CGGACCA20000.015.0774999
GCGCAAG21500.015.0581391
TCGTTTA10950.014.52968130
AGACGGA22250.013.9685396
GGTATCA344250.013.8058091
CAAGACG26800.013.529854
TACCGTC11200.013.3794647
CGCATCG12450.013.37349413
CGAACGT5000.013.324
GCAAGAC30400.013.2664483
ATGGTCG11750.013.22553336
GCGTTAG1409.574524E-613.2142851
TCGAACG4950.013.0808093
TAATACT16800.012.9940474
CGAACGA11400.012.98245616
TGGTCGG13200.012.75378837
Produced by FastQC (version 0.11.5)