FastQCFastQC Report
Thu 28 Apr 2016
ERR1042329.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042329.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23356891
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1249890.5351268711233871No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT999860.4280792336617061No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT989100.4234724561586557No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT547540.23442332286433154No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA360240.1542328557340958No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC349010.1494248528196668No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG308910.13225647197651433No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT267300.1144416009819115No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC247410.10592591282803862No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA245710.10519807623369053No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG234120.100235943216929No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA570350.017.0030671
AAGACGG66800.016.6721555
ACGGACC64450.016.3328158
TCTAGCG40700.016.22727228
CTAGCGG41600.015.96514329
GACGGAC65150.015.9585577
CGCAAGA70150.015.3749092
CAAGACG73750.015.2515264
GCGCAAG74050.015.13977051
CGGACCA71150.014.8728049
CGAACGA46350.014.568516
AGACGGA74300.014.4165546
TATACTG37000.014.25
ACGAACG47800.014.12656915
CGCAATA48100.013.96153736
CGAGCCG71150.013.83274715
GTATTAG68050.013.7832461
ATAACGA49750.013.75879312
TCGTTTA46000.013.67391230
TAACGAA50200.013.56175213
Produced by FastQC (version 0.11.5)