FastQCFastQC Report
Thu 28 Apr 2016
ERR1042328.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042328.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32308266
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1164590.3604619325593023No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT944110.2922193348290496No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT855600.2648238689132991No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT464400.1437403047257318No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT414950.12843462412993628No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC338700.10483385273601499No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA337980.1046109995503937No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA445650.019.8097151
AAGACGG102250.018.0929095
GACGGAC98900.017.789187
ACGGACC99850.017.786688
CGCAAGA109650.016.6019132
AGACGGA109450.016.5139336
CGGACCA107850.016.4330089
CAAGACG121800.016.115354
GCGCAAG118450.015.6340221
TCTAGCG53400.015.34737928
TCGTTTA68400.014.98391830
CTAGCGG56400.014.66223329
TATACTG50800.014.0570875
GCAAGAC154850.013.7988363
CGAACGA54600.013.79029216
GCGAAAG128800.013.63082218
TTAACGG18950.013.56992135
CGAAAGC130100.013.48039919
ACGAACG56000.013.47857115
TACGACG77050.013.4698245
Produced by FastQC (version 0.11.5)