FastQCFastQC Report
Thu 28 Apr 2016
ERR1042327.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042327.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4728664
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT854281.8065990732266026No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT787161.6646562327118186No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT477441.0096720765104055No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT375810.794748791624865No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC141050.29828721177905637No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT117060.24755406601103397No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA115710.24469913700783139No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA99490.21039769372490835No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG98810.2089596554121841No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88480.18711416163212274No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84960.17967019860155004No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG83550.17668838386487176No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA74100.1567038808424536No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66420.1404625069575677No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG65780.13910905913382723No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG62900.13301854392699503No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT59470.12576490949663582No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA50800.1074299210094014No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50610.10702811618672843No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48770.10313695369347452No Hit
GAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAA48200.10193153922545566No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA47550.10055694377946922No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGGC3300.020.18181836
TTAACGG3350.019.88059635
GTCGTAC752.0689818E-417.2666661
ATTAGAG9350.016.422463
CGAACGA6450.015.2015516
CGTCGTA6750.015.07407510
TACCGTC7200.014.9027787
CCGTCGT6800.014.6911769
CGCAAGA11200.014.5357142
ACGGACC10900.014.4266058
ATACCGT8500.014.3647066
AAGACGG11650.014.2918455
ACCGTCG7000.014.2714288
TACGACG7200.014.1319455
GACTTTT20950.014.0405724
GACGGAC11050.013.8959267
ATAATAC6150.013.8373993
TATAGTG3750.013.8133335
CCGATAA7400.013.759
CGGACCA11750.013.6978729
Produced by FastQC (version 0.11.5)