FastQCFastQC Report
Thu 28 Apr 2016
ERR1042326.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042326.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9462527
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT602600.6368277733844246No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT518970.5484475764243526No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT458400.4844371910378697No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT225570.23838241095639673No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA195250.021.4609491
TCGCTAA7750.014.5612914
TTAACGG4200.014.53571435
GTATCAA292100.014.3452592
ACGGACC9950.014.3165838
TATACTG16900.013.79295
TAACGGC4300.013.76744236
GTATTAG26950.013.6604821
TCTATAC13250.013.2641523
TAACGCC6850.013.2335784
CGTATAC2551.6370905E-1113.0588243
CTCGCTA9000.012.9513
CGAACGT3050.012.7377044
GCGGAAT7850.012.72611419
TTAGCGA9200.012.66847927
CGTAGCG7450.012.6644315
TTAGACT11150.012.2780274
TATTAGA18600.012.0349472
TAAGACT15850.012.0220834
TAGCGGA7850.012.01910917
Produced by FastQC (version 0.11.5)