FastQCFastQC Report
Thu 28 Apr 2016
ERR1042325.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042325.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5702408
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT754001.3222484255774052No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT657251.1525832595633283No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT383210.6720143490258852No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT320080.5613067321734958No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA109620.19223457879548428No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT97160.17038416051604865No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC84500.14818301321126093No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73430.12877016165802235No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71560.12549084527098026No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT69240.12142238857689594No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG68220.11963367054760024No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA65470.1148111464490089No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG62070.10884875301802326No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA59450.10425420278591079No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG18450.017.7479675
ACGGACC18600.016.7096778
CGCAAGA19350.016.5400522
GACGGAC18950.016.4010567
TAATACT12100.016.053724
CGGACCA19500.015.8435919
GCGCAAG20850.015.5275771
AGACGGA21100.015.3436026
GGTTATC9850.014.83756352
CAAGACG23050.014.7678964
AGGTTAT10500.014.6238091
GCGAAAG21700.014.578341518
CGAAAGC21600.014.56018519
CAGAGCG22450.014.25612514
CGAACGA8400.014.09523816
ATTAGAG10450.013.9856453
AGCGAAA22400.013.9575917
GTTATCT10900.013.7477063
TTATCTA11300.013.5884954
ATACCGA2051.4279067E-913.5365856
Produced by FastQC (version 0.11.5)