FastQCFastQC Report
Thu 28 Apr 2016
ERR1042324.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042324.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16257062
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1251740.7699669226825856No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1190150.7320818484914433No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1005120.6182666954213498No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT508790.312965528457725No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA557600.015.437681
ACGGACC27550.015.17604358
GACGGAC28300.014.577747
TATACTG20700.013.6739135
GTATTAG41450.013.6127851
AGACGGA32900.013.1580556
CTTATAC70750.012.94346237
AAGACGG37450.012.9425915
CGGACCA32500.012.9215389
TCTTATA108200.012.77218137
TCTAGCG15700.012.49044628
CGAACGA18850.012.36604716
TCTATAC19450.012.2699233
TCGCTAA9350.012.26737914
ATAATAC25700.012.2373533
CGCAAGA35400.012.2288132
GTACTAG8500.012.1882341
ATTAGAC14050.012.1138783
TAACGCC21050.012.040384
ATAACGC26150.012.0267693
Produced by FastQC (version 0.11.5)