FastQCFastQC Report
Thu 28 Apr 2016
ERR1042322.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042322.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6622759
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC496500.7496875546883104No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG396210.598255198475439No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA299250.4518509581882717No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG204620.3089648891043748No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG190530.28768976796528456No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC187370.2829183426423942No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT165160.24938247035714267No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG159810.24130426609212263No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA158490.23931113905851017No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC137020.2068926258678596No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT125460.18943766487652655No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT124820.188471300254169No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT120050.18126886392816044No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT111210.16792095258184694No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT110490.16683379238169468No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC97530.1472649087789545No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC92770.1400775719001703No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA82620.1247516329674687No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG79150.1195121247806239No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT76970.11622044528571854No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT75810.1144689094076955No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA69510.10495625765636345No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTC67480.10189106986982313No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG67190.10145318590031736No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTATA1705.456968E-1217.4117642
TAATACG3300.017.3787884
TTAACGG5250.017.26666635
GTACTAG2600.017.0769231
TAACGGC5750.016.73043436
TATAACG3600.016.4444452
AAGACGG18800.016.4335125
ACGGACC18650.015.7721198
TCTAGCG16200.015.645061528
GACGGAC18450.015.5420067
ATTAGAG15000.015.5400013
TAACGCA4300.015.4883724
CGAACGA11600.015.31034516
CTAGCGG16850.014.9317529
TATTAGA10100.014.8366332
GTTCAAA25000.014.431
TAACGAA12450.014.41365313
CGGACCA20550.014.40389259
TATACTG6700.014.3582095
ACGAACG12700.014.12992115
Produced by FastQC (version 0.11.5)