FastQCFastQC Report
Thu 28 Apr 2016
ERR1042321.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042321.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1023448
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC19990.19532013350946992No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA18220.1780256544543543No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC17000.1661051660660825No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC12610.12321094965254709No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGG10770.10523250814892403No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC10340.1010310245366643No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCTAT701.9224899E-723.7857151
CGACGTT400.00193104323.12500214
GTATAAA705.101021E-621.1428591
CTAGTAC450.00382552520.5555573
ACACTCG450.00382552520.5555576
TCTAGCG1450.020.41379428
AAGACCG555.1425654E-420.1818185
TCTAGAC851.2450346E-619.5882363
TATTAAG701.2190315E-418.52
TTAACGG701.2190315E-418.535
GGCTATA609.235873E-418.51
GTCTAGC500.007034369318.4999981
ATAGACG500.007034369318.49999828
TATACAG1002.874167E-718.4999985
ATACCGT2350.017.3191496
ATACAGT904.445189E-516.4444456
CTAGCGG1801.0913936E-1116.44444529
GGTATCA8350.015.9520961
TACACTC1403.4713594E-815.8571435
TACCGTC2400.015.4166667
Produced by FastQC (version 0.11.5)