FastQCFastQC Report
Thu 28 Apr 2016
ERR1042320.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042320.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1906824
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT592493.1072086359307414No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT494932.595572533175584No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT462772.4269151216892593No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT201291.0556296753135055No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT61440.3222111741828297No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53060.27826375166245026No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49410.2591219745503518No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA45200.23704337684023277No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41680.21858336165267478No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38920.20410903156243052No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA36290.1903164633967267No Hit
CCACAACACAGCTGAGGGCGAAGTGATCACAACCCCCCATGTA35320.18522947057515532No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT34160.17914605647925555No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC33570.176051906206341No Hit
GGTATCAACGCAGAGTACATGGGGGGTTGTGATCACTTCGCCC32710.17154178885938082No Hit
GTATCAACGCAGAGTACATGGGGGGTTGTGATCACTTCGCCCT32560.17075514048491103No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG28210.14794233762528686No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAA27800.14579216540173606No Hit
TATCAACGCAGAGTACATGGGGGGTTGTGATCACTTCGCCCTC27580.1446384144525137No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26620.139603864855907No Hit
ACACAGCTGAGGGCGAAGTGATCACAACCCCCCATGTACTCTG26340.1381354545568967No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25390.13315334818525462No Hit
ACGCAGAGTACATGGGGGGTTGTGATCACTTCGCCCTCAGCTG23360.12250737351743003No Hit
GCTGAGGGCGAAGTGATCACAACCCCCCATGTACTCTGCGTTG22510.11804969939543451No Hit
GTACATGGGAAGCAGTGGTATCAACAAAAAAAAAAAAAAAAAA21540.11296270657386313No Hit
GAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAA19970.10472912025441257No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA19910.10441446090462465No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG19870.10420468800476604No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTATA1201.0913936E-1121.5833322
GTTATAC1850.021.03
ATCGGGT450.003826719520.5555554
GCGTTAT1101.7571438E-920.1818181
GTCGGGA3600.019.013892
TCTTATA15250.018.80327837
ACTATAT2400.018.53
TACCGAC500.00703654118.57
GTACTAT2900.017.8620681
GTCGGTT1451.546141E-1017.86206812
TCGACCG1258.587449E-917.76000218
TAGTGCG752.0681664E-417.2666669
CGCGCAT650.001580450317.07692310
TACGCTA650.001580450317.07692326
CTATATC2750.016.8181824
TCTAACG904.4484303E-516.4444452
TTAGAAC2750.016.1454543
CGGGAGT4300.015.9186044
CTAGATA1751.3278623E-1015.8571434
AACCGCG700.002593333615.8571437
Produced by FastQC (version 0.11.5)