FastQCFastQC Report
Thu 28 Apr 2016
ERR1042319.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042319.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9562246
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54000.018.4657421
TTAACGG5950.015.23529335
TACCGTC14350.014.56794557
ACGGACC17250.014.478268
ATTAGAC8650.014.329483
GACGGAC16950.014.29793557
GTATTAG31850.014.0565141
TAACGCC13700.014.0437964
ATACCGT18000.013.97777756
TCTAGCG12750.013.92941228
TAATACG10150.013.66995054
TCGTTTA12350.013.63157930
ATACCAT17450.013.252156
ACCGTCG15100.013.109278
TATTAGA18350.013.1062672
ATACCGC19950.013.07518827
CCGTCGT15000.013.0733339
TAACGGC7100.013.0281736
CGAGCCG21350.012.99765815
ATAATAC16550.012.9667673
Produced by FastQC (version 0.11.5)