FastQCFastQC Report
Thu 28 Apr 2016
ERR1042318.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042318.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5840552
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT71680.12272812569770802No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT59210.10137740405358946No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT58970.10096648398986946No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35700.020.4173681
TTAACGG2650.016.75471735
TTAGACT7800.015.4166674
TAATACG7350.015.3537414
TAACGCC5000.015.174
ATTAGAC6300.014.976193
TATACCG3600.014.9027785
TATACTG8950.014.8826825
TAACGGC3250.013.66153936
TCTAGAC5700.013.6315793
CTAACGC1501.3079971E-613.5666673
GTACTAG4250.013.4941171
TCTATAC7350.013.3401363
TATTAGA11700.013.2820512
ATTAGAG8700.013.1839083
GTATTAG15650.013.1214061
AATAACG4800.013.1041662
ATACGCC6150.012.9349596
TTATACC12150.012.7901244
ATAATAC12150.012.4855973
Produced by FastQC (version 0.11.5)