FastQCFastQC Report
Thu 28 Apr 2016
ERR1042317.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042317.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5146882
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT215210.41813665050024457No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT183150.3558465105669802No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT172520.3351932296096938No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83340.16192327704423765No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGG5150.017.96116435
TTAGACT5700.017.8508764
GGTATCA95500.017.6282731
ATTAGAC5300.016.405663
TAACGGC5600.016.187536
TGCGACG2750.016.14545422
ACGTTTA4100.015.79268226
TAGGTCG3550.015.63380221
GTACTAG3500.015.3285721
CTAGTAC4350.014.8850583
TCTATAC7550.014.7019873
TCGCTAA8150.014.52760714
TCTATCG4500.014.38888931
TATTAGA14550.014.240552
GTATTAG21700.014.2373281
CTCGCTA8400.014.09523913
CGTCGTA2900.014.03448310
TTTAGCG6750.013.977777526
TAATACG9150.013.950824
CGTAGCG5250.013.74285815
Produced by FastQC (version 0.11.5)