FastQCFastQC Report
Thu 28 Apr 2016
ERR1042316.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042316.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15874128
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT563320.35486673661696566No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT479100.30181185385427156No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT435330.2742386857407223No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT246560.1553219175251705No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA258100.016.7797371
TCTAGCG20250.015.62222128
ACGGACC28100.014.0889688
CTAGCGG22600.013.997786529
CGAACGA18150.013.96418716
GACGGAC27900.013.8584227
GTATTAG47000.013.8159571
TATACTG27150.013.6961335
TTAGACT17450.013.252154
TCGTTTA18600.013.22849530
TTAACGG7150.013.19580535
TATTAGA25800.013.0503872
AAGACGG34550.013.0115775
CGCAATA25500.012.91372636
CGAGCCG36550.012.8563615
GTCCTAT30000.012.5799991
TAATACT36450.012.536354
ATTAGAG27000.012.4018523
TAACGAA20450.012.39364413
GTAAACG24850.012.3581527
Produced by FastQC (version 0.11.5)