FastQCFastQC Report
Thu 28 Apr 2016
ERR1042315.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042315.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8674161
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT747740.8620314979166285No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT641700.7397833634861055No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT607360.7001945202538897No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT274810.31681450229019265No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGG13000.018.64230735
TTAGACT14950.018.5618724
CGTAGCG12350.018.27530315
ATTAGAC13350.018.1535593
TAACGGC13500.017.81481636
TCGTAGC13200.017.6590914
TCTATAC12200.017.1352443
CGGTAAG3350.017.11940428
AATACTG21650.017.004625
TATTAGA31800.016.6383652
ATTAGAG21050.016.4346793
GTACGTA9050.016.3535913
GCGGAAT14150.016.34275619
TAGCGGA13950.016.31182717
TCGGTAA3600.015.93055627
CCGCCTA9300.015.91397915
CGTTCGG3450.015.55072424
CTACCCG9800.015.47959210
GTGACAC17000.015.34411824
TAATACT40750.015.2993864
Produced by FastQC (version 0.11.5)