FastQCFastQC Report
Thu 28 Apr 2016
ERR1042314.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042314.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15510454
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT552450.3561791292505042No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT464450.29944320134020574No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT440990.284317918740483No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT199690.12874542550463064No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGACT21050.019.5106894
GGTATCA208850.019.3370841
ATTAGAC19500.018.4051283
TTAACGG12800.016.62109435
TATTAGA39000.015.9384612
TAGACTA28800.015.9305555
TATACTG17000.015.779415
TAACGGC13750.015.60727236
TCTATAC26000.015.369233
AGACTAT24450.015.3599196
GTATTAG67050.014.4026841
CTAGTAC7900.014.0506323
ATACCAT29750.013.8672276
TCGCTAA12900.013.76744114
CCGCCTA17150.013.69970815
AATACTG30950.013.6882065
ACGGACC22400.013.5446438
ATTAGAG28750.013.5130433
GACTATG29200.013.4315077
TCTAGCG14650.013.25938528
Produced by FastQC (version 0.11.5)