FastQCFastQC Report
Thu 28 Apr 2016
ERR1042313.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042313.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1753867
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC32270.18399342709566915No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA28330.1615287818289528No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC25790.14704649782452148No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT20480.11677054189399767No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC20430.11648545756320176No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC17970.10245930848804385No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG652.6841826E-622.769231
TAACGTT450.003826598920.55555518
TCCTATA500.007036320418.52
TTAACGG701.21966135E-418.535
GACGATA609.239455E-418.513
TACTATA1156.410664E-817.6956522
TATACTG953.6085767E-617.5263165
CTTAACC650.001580381917.0769233
TAAGACT1005.881335E-616.6500024
CGGGAGT4400.016.3977284
GTATAGA1251.6590639E-716.2799991
GTCGGGA4350.016.160922
GTTCAAA5500.016.1454541
ATTAGAG2650.016.0566043
TGCGACG1059.3486215E-615.85714422
GCATTAG700.002593222515.8571421
TTAGAGT2003.6379788E-1215.7254
CTAGTAC957.063061E-515.5789483
GTAAGAC957.063061E-515.5789483
GGTATCA18350.015.5258861
Produced by FastQC (version 0.11.5)