FastQCFastQC Report
Thu 28 Apr 2016
ERR1042312.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042312.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences225111
Sequences flagged as poor quality0
Sequence length43
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTACT358.8471646E-426.428574
GGTATCA856.91216E-1126.1176471
TGCGTTC400.001926392823.12524
TATCCTG400.001926392823.1255
GGACTCT555.125455E-420.1818186
GTATCAA1500.019.7333341
TCCACTA500.00701774818.535
CGGACAT803.3674753E-416.187521
AGGGCCG803.3674753E-416.18755
ACAACAC700.002583820415.8571423
CTGTAAT700.002583820415.8571422
CTCTTAT700.002583820415.85714237
TCCTATT700.002583820415.8571422
CATTAGG700.002583820415.8571421
AAGTTGA700.002583820415.85714229
TCTAAGG957.0194445E-515.57894827
AGGAGTG1101.44150345E-515.1363645
ATTGAGC1101.44150345E-515.1363643
GCAGGAC750.004090787414.83
GTTATGG750.004090787414.81
Produced by FastQC (version 0.11.5)