FastQCFastQC Report
Thu 28 Apr 2016
ERR1042311.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042311.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences523275
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC11250.2149921169557116No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC10400.19874826811905782No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG9970.19053079164875064No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA9250.1767712961635851No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC8980.17161148535664805No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC6970.1331995604605609No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT6880.1314796235249152No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC6790.1297596865892695No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA6260.11963116907935598No Hit
CGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCT5570.10644498590607232No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGG5370.1026229038268597No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTAGA250.00549364229.5999982
GGCTAGC250.00549364229.5999981
ACTAGGG358.8629127E-426.428573
TTAGACC451.3218666E-424.6666664
CAATACT400.001929789423.1254
TTAACGG502.6993774E-422.19999935
TTAAGGC450.003823064520.5555553
TACTAGG450.003823064520.5555552
GTATAGA555.1379483E-420.1818181
CGGGTAA951.6717604E-719.47368424
ATTAGAG951.6717604E-719.4736843
TAACGGC609.227648E-418.536
ATTCCGT1205.155016E-918.529
ATAGTAC609.227648E-418.53
TAGCACA500.007029887318.4999984
GCATTAT500.007029887318.4999981
GCGGGTA1002.8674913E-718.49999823
AACCCGT1552.0008883E-1117.90322729
TATTAGA852.718527E-517.4117642
AGTACTG852.718527E-517.4117645
Produced by FastQC (version 0.11.5)