FastQCFastQC Report
Thu 28 Apr 2016
ERR1042309.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042309.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4766193
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC80700.16931752448967133No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC76150.1597711213121248No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA68950.1446647250751281No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG68550.1438254808397394No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT66490.13950337302748755No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC57880.12143864086074567No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT50290.105513981494245No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC48900.10259760777626924No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCAAA20800.017.2548081
TTAGACT3500.016.9142864
ATTAGAC2500.016.283
ATTAGAG8750.015.8571433
TTAACGG2850.015.57894735
CGAGCCG20850.015.52757715
TAACGGC3000.015.41666836
GTATTAT4250.015.2352941
TTAGAGT6400.015.031254
TCTAGCG11750.014.95744728
ACTCTAA11350.014.83259910
GTCTAAC2006.184564E-1114.81
ATTATAC5550.014.6666673
AGGCCCG22450.014.58574710
TATTAGG3950.014.5189872
TTACGTA1152.2126202E-514.4782614
AAGACGG13300.014.4661655
GACGGAC12150.014.4650217
TTAGCGA2201.8189894E-1114.29545427
CGGGAGT9250.014.24
Produced by FastQC (version 0.11.5)